Dhaliwal JS, Maiselamab Lisut, Lee Yin Yin and Nasuruddin BA
Division of Immunology, Institute for Medical Research, 50588 Kuala Lumpur
CITATION: Dhaliwal JS, Maiselamab Lisut, Lee Yin Yin, Nasuruddin BA. Comparison between serological and molecular typing for HLA Class II antigens (HLA-DR and DQ). International Medical Research Journal. 1998 Jun;2(1):39–42.
ABSTRACT
A total of 17 subjects were typed for HLA-DR Class II antigens using one serologic and two DNA typing kits. Our results showed that DNA typing is superior to serologic typing in assigning the correct HLA type. A total of 8 incorrect assignments and 6 missing assignments were noted. Seven HLA-DQ alleles and 1 HLA-DR8 allele were incorrectly assigned. Discrepancies due to a failure in identifying DR8 (2 cases), DQ6 (2 cases) DR 52 (1 case) and DR 51 (1 case) were also observed.
KEYWORDS: HLA, DNA, PCR
