Monoclonal antibodies against Malaysian Blastocystis hominis isolates

Init I¹, Mak JW², Yong HS^3, Lokman Hakim S¹

1. Division of Parasitology, Institute for Medical Research, 50588 Kuala Lumpur, Malaysia

2. Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Malaysia

3. Department of Zoology, University of Malaya, 59100 Kuala Lumpur, Malaysia

Correspondence: Dr Init I; e-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.

CITATION: Init I, Mak JW, Yong HS, Lokman Hakim S. Monoclonal antibodies against Malaysian Blastocystis hominis isolates. International Medical Research Journal. 1999;3(1):25–32. 

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ABSTRACT

The immunoblotting technique was used with six monoclonal antibodies (MAbs) to analyze the antigenic profiles of 10 Blastocystis hominis isolates [C, H, H6, H7, KP1, Y51, DJ1, RN, 27B05(1), M12] in relation to studies on the pathogenicity of this parasites. MAb RN5-8-5 specifically recognized B. hominis isolated from patients with clinical symptoms while MAbs 4F2-1 and 4C4-2 were also potentially useful for this purpose. The other three MAbs (DJ-9, 9D3-3 and Y44F4) recognized B. hominis isolates recovered from both symptomatic and asymptomatic patients, as well as cross reacted with Escherichia coli and Endolimax nana. All these MAbs did not react with Entamoeba histolytica and Giardia lamblia

KEYWORDS: B. hominis, isolates, monoclonal antibody. immunoblotting