Ezarul Faradianna Lokman
Endocrine and Metabolic Unit, Nutrition, Metabolism and Cardiovascular Research Centre (NMCRC), Institute for Medical Research, National Institutes of Health, Ministry of Health Malaysia, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
Corresponding author:
CITATION: Lokman EF. Guidance protocols to oral glucose tolerance test, pancreatic islets isolation and glucose-stimulated insulin secretion in rats. International Medical Research Journal. 2024 Oct;10(1):39–51.
ABSTRACT
In diabetes research, glucose tolerance tests (GTT) and glucose-stimulated insulin secretion (GSIS) are both established methods to study glucose regulations and insulin release in response to test items. This Protocol will guide researchers in performing in vivo experiments using an oral GTT and ex vivo pancreatic islet isolation to proceed with GSIS procedures in rats. The blood glucose and insulin are measured in response to treatment and glucose administration for GTT. This is followed by an ex vivo experiment to study the GSIS involving Collagenase Digestion Method to obtain isolated pancreatic Islets. Using the overnight incubated pancreatic Islets, GSIS experiment can be performed to determine how well the Islets produce insulin at low and high glucose in response to test items. This Protocol applies to study antidiabetic properties and glucose-lowering effects using rodent models and cell lines. This procedure can also be applied in other rat models, including healthy non-diabetic, diabetic, and diabetic induced rodents.
KEYWORDS: Glucose Tolerance Test, Pancreatic Islets Isolation, Insulin Secretion, Glucose-Stimulated Insulin Secretion
